一种用于各种基质中脂肪酸定量分析的快速方法的评估。
Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices.
作者信息
Araujo Pedro, Nguyen Thu-Thao, Frøyland Livar, Wang Jingdong, Kang Jing X
机构信息
National Institute of Nutrition and Seafood Research, N-5817 Bergen, Norway.
出版信息
J Chromatogr A. 2008 Nov 28;1212(1-2):106-13. doi: 10.1016/j.chroma.2008.10.006. Epub 2008 Oct 8.
Abstract
A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF(3) or BCl(3) in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed.
摘要
提出了一种通过气相色谱对各种基质中的脂肪酸进行定量分析的简化方法，作为传统方法的替代方法，并对该方法的变量进行了研究，以优化处理条件。改进后的方法包括用三卤化硼（甲醇中的BF(3)或BCl(3)）对匀浆样品中的脂肪酸进行直接甲基化，然后用己烷萃取。甲基化过程后向反应混合物中加入己烷可以提高脂肪酸甲基化的效率，对于含有高水平甘油三酯的样品至关重要。提出了这种效应的潜在机制。
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