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本文引用的文献

1
Comparison of four extraction/methylation analytical methods to measure fatty acid composition by gas chromatography in meat.四种用于气相色谱法测定肉类脂肪酸组成的提取/甲基化分析方法的比较
J Chromatogr A. 2008 May 9;1190(1-2):327-32. doi: 10.1016/j.chroma.2008.03.004. Epub 2008 Mar 7.
2
Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues.用于测定海洋组织中脂肪酸和胆固醇的一步提取/甲基化方法的验证
J Chromatogr A. 2006 Feb 3;1104(1-2):291-8. doi: 10.1016/j.chroma.2005.11.045. Epub 2005 Dec 15.
3
A simplified and efficient method for the analysis of fatty acid methyl esters suitable for large clinical studies.一种适用于大型临床研究的、用于分析脂肪酸甲酯的简化高效方法。
J Lipid Res. 2005 Oct;46(10):2299-305. doi: 10.1194/jlr.D500022-JLR200. Epub 2005 Aug 1.
4
Direct methylation from mouse plasma and from liver and brain homogenates.来自小鼠血浆、肝脏和脑组织匀浆的直接甲基化。
Anal Biochem. 2005 Aug 1;343(1):48-53. doi: 10.1016/j.ab.2005.04.037.
5
A simplified method for analysis of polyunsaturated fatty acids.一种分析多不饱和脂肪酸的简化方法。
BMC Biochem. 2005 Mar 24;6:5. doi: 10.1186/1471-2091-6-5.
6
A rapid and quantitative method for total fatty acid analysis of fungi and other biological samples.一种用于真菌及其他生物样品总脂肪酸分析的快速定量方法。
Lipids. 1999 Dec;34(12):1347-9. doi: 10.1007/s11745-999-0488-z.
7
Selection of methylation procedures for quantitation of short-chain glycerol-bound compounds formed during thermoxidation.热氧化过程中形成的短链甘油结合化合物定量分析的甲基化方法选择。
J Chromatogr A. 1999 Nov 26;863(2):171-81. doi: 10.1016/s0021-9673(99)00974-7.
8
Comparison of rapid methods for analysis of bacterial fatty acids.细菌脂肪酸分析快速方法的比较
Appl Microbiol. 1974 Jul;28(1):80-5. doi: 10.1128/am.28.1.80-85.1974.

一种用于各种基质中脂肪酸定量分析的快速方法的评估。

Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices.

作者信息

Araujo Pedro, Nguyen Thu-Thao, Frøyland Livar, Wang Jingdong, Kang Jing X

机构信息

National Institute of Nutrition and Seafood Research, N-5817 Bergen, Norway.

出版信息

J Chromatogr A. 2008 Nov 28;1212(1-2):106-13. doi: 10.1016/j.chroma.2008.10.006. Epub 2008 Oct 8.

DOI:10.1016/j.chroma.2008.10.006
PMID:18937951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2593112/
Abstract

A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF(3) or BCl(3) in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed.

摘要

提出了一种通过气相色谱对各种基质中的脂肪酸进行定量分析的简化方法，作为传统方法的替代方法，并对该方法的变量进行了研究，以优化处理条件。改进后的方法包括用三卤化硼（甲醇中的BF(3)或BCl(3)）对匀浆样品中的脂肪酸进行直接甲基化，然后用己烷萃取。甲基化过程后向反应混合物中加入己烷可以提高脂肪酸甲基化的效率，对于含有高水平甘油三酯的样品至关重要。提出了这种效应的潜在机制。