藤黄八叠球菌产生辅酶A。
Production of coenzyme A by Sarcina lutea.
作者信息
Nishimura N, Shibatani T, Kakimoto T, Chibata I
出版信息
Appl Microbiol. 1974 Jul;28(1):117-23. doi: 10.1128/am.28.1.117-123.1974.
To develop an efficient method for the production of coenzyme A (CoA), optimal conditions for its formation from pantothenic acid, cysteine, and adenine were studied. A number of microorganisms were screened for production of CoA. Strains belonging to the genera Sarcina, Bacillus, Microbacterium, Micrococcus, and Serratia accumulated CoA. Among these, Sarcina lutea was selected as the best organism, and the culture conditions for the production of CoA were investigated with this organism. Under optimal conditions, 600 mug of CoA per ml was accumulated in the culture broth. CoA was readily isolated in high purity by the use of charcoal, diethylaminoethyl-cellulose, Sephadex G-25, and Dowex-50. Yields of isolated CoA were over 33% from culture broth.
为开发一种高效生产辅酶A（CoA）的方法，研究了由泛酸、半胱氨酸和腺嘌呤形成辅酶A的最佳条件。筛选了多种微生物用于生产辅酶A。属于八叠球菌属、芽孢杆菌属、微杆菌属、微球菌属和沙雷氏菌属的菌株积累了辅酶A。其中，藤黄八叠球菌被选为最佳菌株，并以此菌株研究了生产辅酶A的培养条件。在最佳条件下，培养液中每毫升积累了600微克辅酶A。通过使用活性炭、二乙氨基乙基纤维素、葡聚糖G - 25和Dowex - 50可轻松分离出高纯度的辅酶A。从培养液中分离得到的辅酶A产率超过33%。
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