藤黄八叠球菌的一个突变体由核黄素生产黄素腺嘌呤二核苷酸。
Production of flavine-adenine dinucleotide from riboflavine by a mutant of Sarcina lutea.
作者信息
Watanabe T, Uchida T, Kato J, Chibata I
出版信息
Appl Microbiol. 1974 Mar;27(3):531-6. doi: 10.1128/am.27.3.531-536.1974.
A study was made to develop a new method for the production of flavine-adenine dinucleotide (FAD) from riboflavine and adenine by a mutant of Sarcina lutea deficient in the enzyme adenosine deaminase. It was found that this strain could convert exogenously supplemented riboflavine to extracellular FAD. The yields of FAD were increased by addition of D-cycloserine in the culture medium. The culture conditions for FAD production were investigated under the addition of D-cycloserine, and increased production of FAD was observed with the addition of an appropriate amount of thiamine, acetate, and sodium ion. The yield of 0.7 g/liter was obtained in the optimal culture in 5 days. Accumulated FAD was readily isolated by adsorption chromatography and ion-exchange chromatography in a 70% yield.
进行了一项研究，以开发一种新方法，利用一株缺乏腺苷脱氨酶的藤黄八叠球菌突变体，从核黄素和腺嘌呤生产黄素腺嘌呤二核苷酸（FAD）。发现该菌株可将外源添加的核黄素转化为细胞外FAD。通过在培养基中添加D-环丝氨酸提高了FAD的产量。在添加D-环丝氨酸的情况下研究了FAD生产的培养条件，发现添加适量的硫胺素、乙酸盐和钠离子可提高FAD的产量。在最佳培养条件下5天内获得了0.7克/升的产量。通过吸附色谱法和离子交换色谱法可轻松分离积累的FAD，产率为70%。
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