白色八叠球菌产L-丝氨酸。
Production of L-serine by Sarcina albida.
作者信息
Ema M, Kakimoto T, Chibata I
出版信息
Appl Environ Microbiol. 1979 Jun;37(6):1053-8. doi: 10.1128/aem.37.6.1053-1058.1979.
Conditions for the production of microbial L-serine hydroxymethyltransferase and for the conversion of glycine to L-serine were studied. A number of microorganisms were screened for their abilities to form and accululate L-serine from glycine, and Sarcina albida was selected as the best organism. Enzyme activity in this organism as high as 0.12 U/ml could be produced in shaken cultures at 30 degrees C in a medium containing glucose, ammonium sulfate, glycine, yeast extract, and inorganic salts. L-Serine was produced most efficiently by shaking cells at 30 degrees C in a reaction mixture containing 20% glycine, 5 X 10(-3) M formaldehyde, and 3 X 10(-4) M pyridoxal phosphate in yields of 22 mg of broth in 5 days. L-Serine was easily isolated in 84% yields by ion-exchange resin.
对微生物L-丝氨酸羟甲基转移酶的产生条件以及甘氨酸向L-丝氨酸的转化条件进行了研究。筛选了多种微生物从甘氨酸形成并积累L-丝氨酸的能力，选择了白色八叠球菌作为最佳菌株。在含有葡萄糖、硫酸铵、甘氨酸、酵母提取物和无机盐的培养基中，于30℃摇瓶培养时，该菌株的酶活性可达0.12 U/ml。在含有20%甘氨酸、5×10⁻³ M甲醛和3×10⁻⁴ M磷酸吡哆醛的反应混合物中，于30℃振荡培养细胞，5天内每毫升肉汤可高效产生22 mg L-丝氨酸。通过离子交换树脂可轻松分离得到L-丝氨酸，产率为84%。
Zotero 插件