Kirschner L B, Greenwald L, Sanders M
J Gen Physiol. 1974 Aug;64(2):148-65.
Sodium efflux (J(out) (Na)) across the irrigated trout gill was rapid in sea water (SW), but only about 25 % as large in fresh water (FW). The difference correlated with a change in the potential difference across the gill (TEP). The latter was about +10 mV (blood positive) in SW, but -40 mV in FW. Both flux and electrical data indicated that gills in this fish are permeable to a variety of cations including Na(+), K(+), Mg(2+), choline, and Tris. They are less permeable to anions; P(Na):P(K):P(Cl) was estimated to be 1:10:0.3, and P(Cl) > P(gluconate). The TEP was shown to be a diffusion potential determined by these permeabilities and the extant ionic gradients in SW, FW as well as in other media. J(out) (Na) appeared to be diffusive in all of the experiments undertaken. Exchange diffusion need not be posited, and the question of whether there is an active component remains open.
经灌注的虹鳟鱼鳃的钠外流(J(out)(Na))在海水中(SW)很快,但在淡水中(FW)仅约为海水的25%。这种差异与鳃两侧电位差(TEP)的变化相关。后者在海水中约为 +10 mV(血液为正),但在淡水中为 -40 mV。通量和电学数据均表明,这种鱼的鳃对包括Na(+)、K(+)、Mg(2+)、胆碱和三羟甲基氨基甲烷在内的多种阳离子具有通透性。它们对阴离子的通透性较低;P(Na):P(K):P(Cl)估计为1:10:0.3,且P(Cl) > P(葡萄糖酸盐)。TEP被证明是一种由这些通透性以及海水、淡水和其他介质中现存离子梯度所决定的扩散电位。在所有进行的实验中,J(out)(Na)似乎都是扩散性的。无需假定存在交换扩散,并且是否存在主动成分的问题仍未解决。