Zaccone G, Fasulo S, Licata A
Histochemistry. 1984;81(1):47-53. doi: 10.1007/BF00495400.
Cytochemical techniques were used for the light and electron microscopical localization of alkaline phosphatase and potassium-dependent nitrophenyl phosphatase in the epidermal ionocytes of the Teleost Blennius sanguinolentus. The heavier deposition of the reaction products obtained with the different media was shown in the cytoplasmic surface of the labyrinth tubules, the apical vesicles and in intimate association with plasmic membranes. Both plasma membranes and intracellular activities are affected by the addition of specific inhibitors L-p-bromotetramisole oxalate and ouabain) to both complete and control media. The significance of the cytoplasmic localization of both the two enzymes is discussed with reference to current models of transepithelial ion transportation.
采用细胞化学技术对鲈形目鱼类红身鳚表皮离子细胞中的碱性磷酸酶和钾依赖性硝基苯磷酸酶进行光镜和电镜定位。用不同培养基获得的反应产物在迷路小管的细胞质表面、顶端小泡以及与质膜紧密相连处有较重的沉积。在完全培养基和对照培养基中添加特异性抑制剂（L-对溴四咪唑草酸盐和哇巴因）会影响质膜和细胞内活性。参照当前的跨上皮离子转运模型讨论了这两种酶在细胞质中定位的意义。
