Malewicz B, Anderson L E, Crilly K, Jenkin H M
In Vitro Cell Dev Biol. 1985 Aug;21(8):470-6. doi: 10.1007/BF02620836.
Serum-free media were developed to grow diploid fetal rhesus monkey lung (DBS-FRhL-2) cells and to propagate dengue-type 2 virus vaccine strain PR-159 (dengue-2 vaccine virus). Vitamins, amino acids, growth factors, hormones and other organic compounds, and inorganic salts were substituted for fetal bovine serum. The composition of the medium that was optimal for growth of DBS-FRhL-2 cells differed from medium optimal for the propagation of dengue-2 vaccine virus. Insulin, epidermal growth factor, fibroblast growth factor, and platelet-derived growth factor were required for DBS-FRhL-2 cell proliferation in serum-free medium but were inhibitory for virus propagation. Adenosine, cytidine, guanosine, uridine, and thymidine, each at 0.01 mM concentration, were necessary as medium supplements to obtain a high yield of dengue-2 vaccine virus in DBS-FRhL-2 cells under serum-free conditions. DBS-FRhL-2 cells grown in serum-free medium produced dengue-2 vaccine virus with yields similar to those of cells grown in the presence of serum. Dengue-2 vaccine virus obtained under serum-free conditions retained its phenotypic markers such as temperature sensitivity and small plaque size.
无血清培养基被开发用于培养恒河猴二倍体胎肺（DBS-FRhL-2）细胞以及繁殖登革2型病毒疫苗株PR-159（登革2疫苗病毒）。维生素、氨基酸、生长因子、激素和其他有机化合物以及无机盐被用来替代胎牛血清。最适合DBS-FRhL-2细胞生长的培养基成分与最适合登革2疫苗病毒繁殖的培养基不同。胰岛素、表皮生长因子、成纤维细胞生长因子和血小板衍生生长因子是无血清培养基中DBS-FRhL-2细胞增殖所必需的，但对病毒繁殖有抑制作用。腺苷、胞苷、鸟苷、尿苷和胸苷，每种浓度为0.01 mM，作为培养基补充剂是在无血清条件下在DBS-FRhL-2细胞中获得高产登革2疫苗病毒所必需的。在无血清培养基中生长的DBS-FRhL-2细胞产生的登革2疫苗病毒产量与在有血清存在下生长的细胞相似。在无血清条件下获得的登革2疫苗病毒保留了其表型特征，如温度敏感性和小蚀斑大小。
