登革病毒在无血清培养基中培养的多倍体蚊细胞培养物中的复制。
Dengue virus replication in a polyploid mosquito cell culture grown in serum-free medium.
作者信息
Kuno G
出版信息
J Clin Microbiol. 1982 Nov;16(5):851-5. doi: 10.1128/jcm.16.5.851-855.1982.
Abstract
A subline of a polyploid cell line (TRA-284) derived from a nonbiting mosquito, Toxorhynchites amboinensis, was adapted to a serum-free medium. The sensitivity of the subline (TRA-284-SF) to all serotypes of adapted dengue viruses was generally comparable to that of Aedes albopictus (C6/36), and DEN 3 viruses replicated to higher titers in TRA-28F-SF cells than in C6/36 cells. The subline was found to be useful for isolation of dengue viruses from human serum, since isolation rates were higher in TRA-284-SF cells than in C6/36 cells. The advantages of using a serum-free medium and mosquito cells for virus isolation are discussed.
摘要
从非吸血蚊种——amboinensis巨蚊中获得的多倍体细胞系（TRA - 284）的一个亚系被适应于无血清培养基。该亚系（TRA - 284 - SF）对所有血清型的适应登革病毒的敏感性总体上与白纹伊蚊（C6/36）相当，并且DEN 3病毒在TRA - 28F - SF细胞中复制到的滴度高于在C6/36细胞中的滴度。发现该亚系对于从人血清中分离登革病毒很有用，因为在TRA - 284 - SF细胞中的分离率高于在C6/36细胞中的分离率。本文讨论了使用无血清培养基和蚊细胞进行病毒分离的优点。
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