金黄色葡萄球菌中的自溶作用：旧细胞壁的优先释放。
Autolysis in Staphylococcus aureus: preferential release of old cell walls.
作者信息
Gilpin R W, Narrod S, Wong W, Young F E, Chatterjee A N
出版信息
J Bacteriol. 1974 Sep;119(3):672-6. doi: 10.1128/jb.119.3.672-676.1974.
Abstract
The kinetics of release of old versus new cell wall in two strains of Staphylococcus aureus were studied during autolysis. In both strains the autolytic enzyme is an amidase. Cells were double labeled with (3)H and (14)C, and the distribution of radioactivity in the cell walls was monitored during autolysis. In all cases the rate of release of steady-state lable from peptidoglycan was significantly higher than that of pulse label. Identical results were obtained with whole cells or isolated cell walls. The results suggest that in S. aureus the old cell wall is preferentially released during autolysis.
摘要
在自溶过程中，研究了两株金黄色葡萄球菌中旧细胞壁与新细胞壁释放的动力学。在这两株菌中，自溶酶均为酰胺酶。细胞用³H和¹⁴C进行双重标记，并在自溶过程中监测细胞壁中放射性的分布。在所有情况下，肽聚糖中稳态标记的释放速率均显著高于脉冲标记的释放速率。完整细胞或分离的细胞壁均得到相同结果。这些结果表明，在金黄色葡萄球菌中，自溶过程中旧细胞壁优先被释放。
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