Ward J B, Perkins H R
Biochem J. 1974 Jun;139(3):781-4. doi: 10.1042/bj1390781.
A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by (14)C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.
来自地衣芽孢杆菌突变体的壁加膜制剂掺入了来自肽聚糖前体的放射性,其中二氨基庚二酸的游离氨基被(14)C标记的乙酰基阻断。这种掺入对青霉素敏感。酶降解产物含有交联二聚体,表明新合成的肽聚糖链已与预先存在的细胞壁交联。