来自灰色链霉菌的两种溶葡萄球菌酶的纯化及性质

Ward J B, Perkins H R

Biochem J. 1968 Jan;106(1):69-76. doi: 10.1042/bj1060069.

Two staphylolytic enzymes have been purified from cultures of a soil isolate of Streptomyces griseus. 2. The purified enzymes were shown to be basic proteins of low molecular weight. Each enzyme released N-acetylmuramic acid reducing groups from the cell walls of Staphylococcus aureus. 3. The enzymes lysed whole staphylococci best at higher pH values and lower ionic strengths than when the substrate was isolated cell walls or purified mucopeptide. 4. Added teichoic acid did not inhibit the enzymes, but it formed an ethanol-precipitable complex with them. 5. The possibility that teichoic acid on the surface of whole cells prevents the access of the enzymes to their mucopeptide substrate is discussed.

已从灰色链霉菌土壤分离株的培养物中纯化出两种溶葡萄球菌酶。2. 纯化后的酶显示为低分子量的碱性蛋白质。每种酶都能从金黄色葡萄球菌的细胞壁释放出N - 乙酰胞壁酸还原基团。3. 与底物为分离的细胞壁或纯化的粘肽相比，这些酶在较高pH值和较低离子强度下对完整葡萄球菌的裂解效果最佳。4. 添加的磷壁酸不会抑制这些酶，但会与它们形成乙醇可沉淀的复合物。5. 讨论了全细胞表面的磷壁酸阻止酶接近其粘肽底物的可能性。

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核心技术专利：CN118964589B侵权必究

Ward J B, Perkins H R

Biochem J. 1968 Jan;106(1):69-76. doi: 10.1042/bj1060069.

Two staphylolytic enzymes have been purified from cultures of a soil isolate of Streptomyces griseus. 2. The purified enzymes were shown to be basic proteins of low molecular weight. Each enzyme released N-acetylmuramic acid reducing groups from the cell walls of Staphylococcus aureus. 3. The enzymes lysed whole staphylococci best at higher pH values and lower ionic strengths than when the substrate was isolated cell walls or purified mucopeptide. 4. Added teichoic acid did not inhibit the enzymes, but it formed an ethanol-precipitable complex with them. 5. The possibility that teichoic acid on the surface of whole cells prevents the access of the enzymes to their mucopeptide substrate is discussed.

已从灰色链霉菌土壤分离株的培养物中纯化出两种溶葡萄球菌酶。2. 纯化后的酶显示为低分子量的碱性蛋白质。每种酶都能从金黄色葡萄球菌的细胞壁释放出N - 乙酰胞壁酸还原基团。3. 与底物为分离的细胞壁或纯化的粘肽相比，这些酶在较高pH值和较低离子强度下对完整葡萄球菌的裂解效果最佳。4. 添加的磷壁酸不会抑制这些酶，但会与它们形成乙醇可沉淀的复合物。5. 讨论了全细胞表面的磷壁酸阻止酶接近其粘肽底物的可能性。