Perkins H R
Biochem J. 1969 Jan;111(2):195-205. doi: 10.1042/bj1110195.
Vancomycin and ristocetin formed complexes on being mixed with mucopeptide precursors from various bacteria, as shown by chromatography, electrophoresis and differential ultraviolet spectra. Equimolar proportions of antibiotic and peptide were present. The specificity of the reaction was studied and the smallest molecule found to react was acetyl-d-alanyl-d-alanine. This C-terminal dipeptide sequence must be present for complex-formation; change of configuration or esterification prevented it. Modified vancomycins that retained antibiotic activity also combined with appropriate peptides. The dissociation constants of the more stable complexes were estimated from the differential-absorption results. The relationship of complex-formation to antibiotic action is discussed. Penicillin, supposed to be an analogue of acyl-d-alanyl-d-alanine, also modified the spectrum of vancomycin; so, too, did sodium benzylpenicilloate.
经色谱法、电泳法和差示紫外光谱法表明，万古霉素和瑞斯托菌素与来自各种细菌的粘肽前体混合时会形成复合物。抗生素和肽以等摩尔比例存在。研究了该反应的特异性，发现能发生反应的最小分子是乙酰基 -d-丙氨酰 -d-丙氨酸。形成复合物必须存在这个C末端二肽序列；构型改变或酯化会阻止复合物形成。保留抗生素活性的修饰万古霉素也能与适当的肽结合。根据差示吸收结果估算了更稳定复合物的解离常数。讨论了复合物形成与抗生素作用的关系。被认为是酰基 -d-丙氨酰 -d-丙氨酸类似物的青霉素，也改变了万古霉素的光谱；苄青霉素钠也有同样的作用。
