使用放射性标记的T1核糖核酸酶来监测埃德曼降解法的效率。
The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations.
作者信息
McKean D J, Smith G P
出版信息
Biochem J. 1974 Jun;139(3):779-80. doi: 10.1042/bj1390779.
Abstract
A simple radiolabel tracer method is presented for monitoring the efficiency of the first ten cycles of an Edman degradation. This method consists of adding a very small amount of radiolabelled S-carboxymethylated T(1) ribonuclease to the protein to be sequenced, and counting the radioactivity of a small sample from each of the degradative cycles.
摘要
本文介绍了一种简单的放射性标记示踪法，用于监测埃德曼降解法前十轮循环的效率。该方法包括向待测序的蛋白质中加入极少量的放射性标记的S-羧甲基化T(1)核糖核酸酶，并对每个降解循环的一小份样品进行放射性计数。
相似文献
1
The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations.使用放射性标记的T1核糖核酸酶来监测埃德曼降解法的效率。
Biochem J. 1974 Jun;139(3):779-80. doi: 10.1042/bj1390779.
2
The structure and function of ribonuclease T1. XI. Modification of the single arginine residue in ribonuclease T1 by phenylglyoxal and glyoxal.核糖核酸酶T1的结构与功能。XI. 苯乙二醛和乙二醛对核糖核酸酶T1中单个精氨酸残基的修饰。
J Biochem. 1970 Nov;68(5):659-64. doi: 10.1093/oxfordjournals.jbchem.a129399.
3
[Proceedings: Catalytic titration in solution of ribonuclease and oxidized ribonuclease. Distribution of radioactivity among the amino acids].
Arch Int Physiol Biochim. 1975 May;83(2):391-3.
4
The amino acid sequence of dromedary pancreatic ribonuclease.单峰骆驼胰核糖核酸酶的氨基酸序列。
Biochem J. 1975 Jun;147(3):505-11. doi: 10.1042/bj1470505.
5
Studies on the photooxidation of Ribonuclease T1.
J Biochem. 1970 Jul;68(1):69-79.
6
Purification of a corn ribonuclease.一种玉米核糖核酸酶的纯化
J Biol Chem. 1967 May 10;242(9):2260-3.
7
Ribonuclease T1, Structure and function.核糖核酸酶T1，结构与功能。
Adv Biophys. 1970;1:53-98.
8
Action of dimeric hybrids of native and selectively alkylated ribonuclease A on double-stranded ribonucleic acid.天然及选择性烷基化核糖核酸酶A的二聚体杂合体对双链核糖核酸的作用。
Biochem J. 1974 Jul;141(1):317-20. doi: 10.1042/bj1410317.
9
Inactivation of ribonuclease by the primary aqueous radicals.核糖核酸酶被初级水合自由基灭活。
Radiat Res. 1972 Dec;52(3):588-602.
10
A modified method for determining hydrogen-deuterium exchange in proteins.
Biochim Biophys Acta. 1970 Sep 29;214(3):551-3. doi: 10.1016/0005-2795(70)90316-8.
引用本文的文献
1
Isolation and partial characterization of human parotid basic proteins.人腮腺碱性蛋白的分离与部分特性鉴定
Biochem Genet. 1977 Oct;15(9-10):925-46. doi: 10.1007/BF00483989.
2
Mechanisms of antibody diversity: multiple genes encode structurally related mouse kappa variable regions.抗体多样性的机制：多个基因编码结构相关的小鼠κ轻链可变区。
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3913-7. doi: 10.1073/pnas.75.8.3913.
本文引用的文献
1
The structure and function of ribonuclease T 1 . XVII. Isolation and amino acid sequences of papain and subtilisin peptides from ribonuclease T 1 --the complete covalent structure of ribonuclease T 1 .核糖核酸酶T1的结构与功能。十七。来自核糖核酸酶T1的木瓜蛋白酶和枯草杆菌蛋白酶肽段的分离及氨基酸序列——核糖核酸酶T1的完整共价结构。
J Biochem. 1971 Dec;70(6):945-60. doi: 10.1093/oxfordjournals.jbchem.a129724.
2
Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.用于埃德曼-贝格蛋白质测序仪的定量方法。两种异常免疫球蛋白轻链Rzf和Sac的部分序列。
Biochemistry. 1971 Dec 21;10(26):4912-21. doi: 10.1021/bi00802a013.
Zotero 插件