使用放射性标记的T1核糖核酸酶来监测埃德曼降解法的效率。
The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations.
作者信息
McKean D J, Smith G P
出版信息
Biochem J. 1974 Jun;139(3):779-80. doi: 10.1042/bj1390779.
Abstract
A simple radiolabel tracer method is presented for monitoring the efficiency of the first ten cycles of an Edman degradation. This method consists of adding a very small amount of radiolabelled S-carboxymethylated T(1) ribonuclease to the protein to be sequenced, and counting the radioactivity of a small sample from each of the degradative cycles.
摘要
本文介绍了一种简单的放射性标记示踪法，用于监测埃德曼降解法前十轮循环的效率。该方法包括向待测序的蛋白质中加入极少量的放射性标记的S-羧甲基化T(1)核糖核酸酶，并对每个降解循环的一小份样品进行放射性计数。
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本文引用的文献
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The structure and function of ribonuclease T 1 . XVII. Isolation and amino acid sequences of papain and subtilisin peptides from ribonuclease T 1 --the complete covalent structure of ribonuclease T 1 .核糖核酸酶T1的结构与功能。十七。来自核糖核酸酶T1的木瓜蛋白酶和枯草杆菌蛋白酶肽段的分离及氨基酸序列——核糖核酸酶T1的完整共价结构。
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