Peters E H, Azen E A
Biochem Genet. 1977 Oct;15(9-10):925-46. doi: 10.1007/BF00483989.
Methods are presented for the isolation of basic proteins (Pb proteins) from human parotid saliva collected from humans possessing different alleles at the Pb locus. The proteins were found to be extremely basic, with an isoelectric point above 9.5. They contain approximately 45% of the basic amino acids histidine, lysine, and arginine, and are devoid of cysteine, proline, threonine, valine, methionine, and tryptophan. They are free of carbohydrate. A comparison of the amino acid sequence data of Pb protein to all available amino acid sequences revealed that no sequence similarities exist between the Pb proteins and any other proteins reported, although proteins of similar amino acid compositions have been reported by others. A model is presented with accounts for the several forms of allelic proteins based on observed amino acid sequence differences.
本文介绍了从具有铅（Pb）位点不同等位基因的人类腮腺唾液中分离碱性蛋白（Pb蛋白）的方法。发现这些蛋白具有极高的碱性，其等电点高于9.5。它们含有约45%的碱性氨基酸组氨酸、赖氨酸和精氨酸，且不含半胱氨酸、脯氨酸、苏氨酸、缬氨酸、蛋氨酸和色氨酸。它们不含碳水化合物。将Pb蛋白的氨基酸序列数据与所有可用的氨基酸序列进行比较后发现，尽管其他人曾报道过氨基酸组成相似的蛋白，但Pb蛋白与其他任何已报道的蛋白之间不存在序列相似性。基于观察到的氨基酸序列差异，提出了一个解释等位基因蛋白多种形式的模型。
