Schmidtke J R, Simmons R L
J Natl Cancer Inst. 1975 Jun;54(6):1379-84. doi: 10.1093/jnci/54.6.1379.
Neuraminidase from Vibrio cholerae (VCN) was used to treat sheep red blood cells (SRBC) which were then incubated in vitro with murine peritoneal macrophages. The uptake of VCN-treated SRBC by macrophages was greater than the uptake of SRBC not treated with VCN. SRBC opsonized with normal mouse serum (NMS) were taken up to a greater extent than untreated SRBC. SRBC treated with VCN and opsonized with NMS were phagocytosed to a greater extent than untreated SRBC, VCN-treated SRBC, or opsonized SRBC. Evidence demonstrated that factors in serum from normal C3H/HeJ mice augmented the uptake of VCN-treated SRBC in greater amounts than of normal SRBC. These findings were discussed in relation to the increased immunogenicity of neuraminidase-treated cells.
霍乱弧菌神经氨酸酶（VCN）被用于处理绵羊红细胞（SRBC），随后将其与小鼠腹腔巨噬细胞进行体外培养。巨噬细胞对经VCN处理的SRBC的摄取量大于对未经VCN处理的SRBC的摄取量。用正常小鼠血清（NMS）调理过的SRBC比未处理的SRBC摄取程度更高。经VCN处理并用NMS调理的SRBC比未处理的SRBC、经VCN处理的SRBC或经调理的SRBC的吞噬程度更高。有证据表明，正常C3H/HeJ小鼠血清中的因子增强了对经VCN处理的SRBC的摄取量，且比对正常SRBC的摄取量增加得更多。结合神经氨酸酶处理细胞免疫原性增加的情况对这些发现进行了讨论。
