Rhodes D P, Abraham G, Colonno R J, Jelinek W, Banerjee A K
J Virol. 1977 Mar;21(3):1105-12. doi: 10.1128/JVI.21.3.1105-1112.1977.
The smallest size class of mRNA (12S) synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus contains two mRNA species of similar molecular weight that code for the viral M and NS proteins. The resolution of these mRNA species was achieved by converting them to duplexes by annealing with the genome RNA, followed by RNase T2 treatment and separation in a polyacrylamide gel. Using this separation technique, the mRNA's were identified by comparing the relative resistance of their syntheses to UV irradiation of the virus. The molecular weights of these two mRNA species calculated as duplex RNAs were smaller than expected. The possible reasons for this discrepancy are discussed.
水泡性口炎病毒的病毒粒子相关RNA聚合酶在体外合成的最小尺寸类别的mRNA（12S）包含两种分子量相似的mRNA种类，它们编码病毒的M蛋白和NS蛋白。通过与基因组RNA退火将这些mRNA种类转化为双链体，随后进行RNase T2处理并在聚丙烯酰胺凝胶中分离，从而实现了这些mRNA种类的分离。使用这种分离技术，通过比较它们的合成对病毒紫外线照射的相对抗性来鉴定mRNA。计算为双链RNA的这两种mRNA种类的分子量小于预期。讨论了这种差异的可能原因。
