Mansueto S, Picone D, La Cascia C, Di Rosa S
Ann Sclavo. 1979 Jan-Feb;21(1):93-9.
Technique of counter immunoelectrophoresis (CIEP) was employed for the diagnosis of V.L. (human and canine) using strips of cellulose acetate and an antigen grossly extracted (by means of repeated freezing and thawing) from culture of Leishmania. 4 lots of antigen was prepared, in various time, at the same way. Positive results were obtained in V.L. from 81 to 90% (according to various lots of antigen). False positivities (from 1 to 4.5%) occur in patient with other diseases (especially cirrhosis and blood disorders). None positivity in controls (blood donors). Present results and those obtained with same technique in agar, suggested the validity of method and encouraged the production of antigen in a "kit" form for use in the field.
采用对流免疫电泳（CIEP）技术，使用醋酸纤维素条带和从利什曼原虫培养物中通过反复冻融粗提取的抗原，对人类和犬类的内脏利什曼病进行诊断。以相同方式在不同时间制备了4批抗原。内脏利什曼病的阳性率为81%至90%（根据不同批次的抗原）。其他疾病患者（尤其是肝硬化和血液疾病患者）出现假阳性（1%至4.5%）。对照组（献血者）均为阴性。目前的结果以及用相同技术在琼脂中获得的结果表明该方法有效，并鼓励以“试剂盒”形式生产抗原以供现场使用。
