Rehewy M S, Hafez E S, Thomas A, Brown W J
Arch Androl. 1979 May;2(3):263-8. doi: 10.3109/01485017908987323.
Semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group A) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group B). These 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. Overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group A were positive and 73% from group B were positive. Mixed bacterial flora were isolated from both groups but in group B they were more varied and present in a higher colony count than in group A. Staphylococcus epidermidis, Staph. aureus, Corynebacterium species, Mycoplasma hominis, and Ureaplasma urealyticum were isolated from group A. Group B revealed these organisma plus Streptococcus pneumoniae type III, Strep. pyogenes group A, Strep. feacalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans, Bacteroides species, Peptostreptococcus species, and Eubacterium species. Semen samples from group A were of better quality than those from group B. In addition, the antibacterial effect of seminal plasma from group A was greater than that from group B.
精液样本通过手淫在无菌条件下采集，采集对象为过去6个月内有生育子女的男性（A组）以及前往不孕不育诊所就诊的无症状男性，这些男性未生育子女且其妻子无不孕症状（B组）。对这109份精液样本进行分析并培养，以分离需氧菌和厌氧菌。总体而言，68%的标本细菌培养呈阳性：A组54%的样本呈阳性，B组73%的样本呈阳性。两组均分离出混合菌群，但B组的菌群种类更多，菌落计数也高于A组。从A组分离出表皮葡萄球菌、金黄色葡萄球菌、棒状杆菌属、人型支原体和解脲脲原体。B组除了这些微生物外，还分离出Ⅲ型肺炎链球菌、A组化脓性链球菌、粪肠球菌、大肠埃希菌、肺炎克雷伯菌、奇异变形杆菌、铜绿假单胞菌、白色念珠菌、拟杆菌属、消化链球菌属和真杆菌属。A组精液样本的质量优于B组。此外，A组精浆的抗菌效果大于B组。
