Barkulis S S, Boltralik J J, Hankin H, Heymann H
J Bacteriol. 1967 Oct;94(4):963-5. doi: 10.1128/jb.94.4.963-965.1967.
Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for acetylglucosamine in providing the C1-C6 moiety of muramic acid. Although radioactivity in the C2-C9 portion of muramic acid suggested that some of the lactyl group (C7-C9) came from glycolytic products, there was also considerable contribution to it from noncarbohydrate sources. Using cell-free extracts, we were unable to demonstrate biosynthesis of acetylmuramic acid, either free or nucleotide-bound, while glycolysis was occurring. The formation of uridine diphosphoacetylmuramic acid has been reported by others who used uridine diphospho-N-acetyl-d-glucosamine, phosphoenolpyruvate, reduced nicotinamide adenine dinucleotide, and reduced nicotinamide adenine dinucleotide phosphate. However, we did not detect the formation of this compound.
将葡萄糖 -1-(14)C 和 N - 乙酰葡糖胺 -1-(14)C 单独添加，并与等量的未标记的对应物一起加入脑心浸液中，用于化脓性链球菌的培养。细胞壁中鼠李糖、葡糖胺和胞壁酸的标记模式支持 N - 乙酰葡糖胺在提供胞壁酸的 C1 - C6 部分中起中间作用。虽然胞壁酸 C2 - C9 部分的放射性表明一些乳酰基（C7 - C9）来自糖酵解产物，但非碳水化合物来源对其也有相当大的贡献。在糖酵解发生时，使用无细胞提取物，我们无法证明游离或核苷酸结合形式的乙酰胞壁酸的生物合成。其他人使用尿苷二磷酸 -N - 乙酰 -d - 葡糖胺、磷酸烯醇丙酮酸、还原型烟酰胺腺嘌呤二核苷酸和还原型烟酰胺腺嘌呤二核苷酸磷酸报道了尿苷二磷酸乙酰胞壁酸的形成。然而，我们没有检测到该化合物的形成。
