自旋回波核磁共振在全细胞系统中的应用。膜运输。
Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.
作者信息
Brindle K M, Brown F F, Campbell I D, Grathwohl C, Kuchel P W
出版信息
Biochem J. 1979 Apr 15;180(1):37-44. doi: 10.1042/bj1800037.
Abstract
A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.
摘要
本文介绍了一种研究膜转运的新方法。高分辨率核磁共振用于测量小分子在细胞内和细胞外区室之间的分布。该方法采用自旋回波技术，依赖于细胞内外介质磁化率的差异。它还能同时提供有关细胞代谢状态的信息。通过对人红细胞中丙氨酸和乳酸转运的研究对该方法进行了说明。
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