红细胞和蛋白质催化丙酮酸-水同位素交换的研究。
Studies of pyruvate-water isotope exchange catalysed by erythrocytes and proteins.
作者信息
Simpson R J, Brindle K M, Brown F F, Campbell I D, Foxall D L
出版信息
Biochem J. 1981 Feb 1;193(2):401-6. doi: 10.1042/bj1930401.
Abstract
Erythrocyte suspensions in buffer made with 2H2O catalyse the exchange of pyruvate protons. This process can be easly observed by spin-echo proton magnetic resonance. The dominant exchange process is shown to be due to the formation of Schiff-base links between pyruvate and amino groups of haemoglobin. Other proteins with free alpha-amino groups also catalyse the exchange. The pH*-dependence of the exchange rate due to hen-egg-white-lysozyme reflects the dissociation of the alpha-amino group.
摘要
用重水（2H₂O）配制的缓冲液中的红细胞悬液可催化丙酮酸质子的交换。这个过程可以通过自旋回波质子磁共振轻松观察到。结果表明，主要的交换过程是由于丙酮酸与血红蛋白的氨基之间形成了席夫碱连接。其他具有游离α-氨基的蛋白质也能催化这种交换。由于鸡蛋清溶菌酶导致的交换速率对pH*的依赖性反映了α-氨基的解离。
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