亲脂性醌与反硝化副球菌和表皮葡萄球菌膜片段的相互作用。
Interaction of lipophilic quinones with membrane fragments of Paracoccus denitrificans and Staphylococcus epidermidis.
作者信息
Mikes V, Dadák V
出版信息
Biochem J. 1979 Apr 15;180(1):69-73. doi: 10.1042/bj1800069.
In quinone-depleted mitochondrial and Paracoccus denitrificans membranes the quantum yield of fluorescence of ostruthin (6-geranyl-7-hydroxycoumarin) was maintained, whereas an increase in the quantum yield took place after extraction of Staphylococcus epidermidis membrane. A marked quenching effect of ubiquinone and menaquinone each with two isoprene units in the side chain on the ostruthin fluorescence was found with all types of quinone-depleted particles. When the homogues of menaquinone and ubiquinone with six isoprene units in the side chain were re-incorporated, a quenching of the ostruthin fluorescence was observed in the S. epidermidis membranes but not in those of P. denitrificans. The different behaviour of both bacterial preparations is attributable to the more specific finding of ubiquinone in the particles of P. denitrificans.
在醌耗尽的线粒体膜和反硝化副球菌膜中，欧芹素（6 - 香叶基 - 7 - 羟基香豆素）的荧光量子产率得以维持，而在提取表皮葡萄球菌膜后荧光量子产率增加。在所有类型的醌耗尽颗粒中，均发现侧链带有两个异戊二烯单元的泛醌和甲基萘醌对欧芹素荧光有显著的猝灭作用。当重新掺入侧链带有六个异戊二烯单元的甲基萘醌和泛醌同系物时，在表皮葡萄球菌膜中观察到欧芹素荧光猝灭，而在反硝化副球菌膜中未观察到。两种细菌制剂的不同行为归因于在反硝化副球菌颗粒中更特异性地发现泛醌。
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