再生大鼠肝脏中的半乳糖代谢
Galactose metabolism in regenerating rat liver.
作者信息
Bauer C H, Hassels B F, Reutter W G
出版信息
Biochem J. 1976 Jan 15;154(1):141-7. doi: 10.1042/bj1540141.
Abstract
- Rats trained on a controlled lighting and feeding schedule were subjected to partial hepatectomy or sham operation. 2. After a large period of about 6h the activity of UDP-galactose 4-epimerase increased threefold, reaching a maximum 4 days after partial hepatectomy, and returned to normal values within a fortnight. 3. The enzyme pattern of the UDP-galactose-glycoprotein galactosyltransferase was biphasic, one peak appearing at 20 h, the second at 72 h after partial hepatectomy. 4. The rise in enzyme activities could be blocked by the injection of actinomycin D, and the Km values for UDP-glucose and UDP-galactose were nearly identical in regenerating and adult liver. It is therefore concluded that the increase in enzyme activity is due to synthesis de novo of enzyme protein.
摘要
- 对按照可控光照和进食时间表训练的大鼠进行部分肝切除术或假手术。2. 大约6小时的较长时间段后，UDP-半乳糖4-表异构酶的活性增加了两倍，在部分肝切除术后4天达到最大值，并在两周内恢复到正常值。3. UDP-半乳糖-糖蛋白半乳糖基转移酶的酶谱呈双相性，一个峰值出现在部分肝切除术后20小时，第二个峰值出现在72小时。4. 注射放线菌素D可阻断酶活性的升高，再生肝和成年肝中UDP-葡萄糖和UDP-半乳糖的Km值几乎相同。因此得出结论，酶活性的增加是由于酶蛋白的从头合成。
相似文献
1
Galactose metabolism in regenerating rat liver.再生大鼠肝脏中的半乳糖代谢
Biochem J. 1976 Jan 15;154(1):141-7. doi: 10.1042/bj1540141.
2
Enzymatic synthesis and interconversion of UDP-D-glucose and UDP-D-galactose in the albumen gland of the snail, Helix pomatia.蜗牛（苹果蜗牛）蛋白腺中UDP-D-葡萄糖和UDP-D-半乳糖的酶促合成与相互转化
Comp Biochem Physiol B. 1976;54(1):135-9. doi: 10.1016/0305-0491(76)90070-5.
3
Changing the donor cofactor of bovine alpha 1, 3-galactosyltransferase by fusion with UDP-galactose 4-epimerase. More efficient biocatalysis for synthesis of alpha-Gal epitopes.通过与UDP-半乳糖4-表异构酶融合改变牛α1,3-半乳糖基转移酶的供体辅因子。用于α-Gal表位合成的更高效生物催化。
J Biol Chem. 2000 Oct 13;275(41):31594-600. doi: 10.1074/jbc.M004005200.
4
UDP-Glucose 4-Epimerase and β-1,4-Galactosyltransferase from the Oyster as Valuable Biocatalysts for the Production of Galactosylated Products.牡蛎中的 UDP-葡萄糖 4-差向异构酶和β-1,4-半乳糖基转移酶作为生产半乳糖基化产物的有价值的生物催化剂。
Int J Mol Sci. 2018 May 29;19(6):1600. doi: 10.3390/ijms19061600.
5
Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coli.来自大肠杆菌的UDP-半乳糖4-差向异构酶对UDP-半乳糖和UDP-葡萄糖的结合存在显著差异。
Biochemistry. 1998 Aug 18;37(33):11469-77. doi: 10.1021/bi9808969.
6
Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli.大肠杆菌UDP-半乳糖4-差向异构酶与UDP-糖结合的结构分析。
Biochemistry. 1997 May 27;36(21):6294-304. doi: 10.1021/bi970025j.
7
Immobilization of UDP-galactose 4-epimerase from Escherichia coli on the yeast cell surface.将来自大肠杆菌的UDP-半乳糖4-表异构酶固定在酵母细胞表面。
Biosci Biotechnol Biochem. 2006 Sep;70(9):2303-6. doi: 10.1271/bbb.60134. Epub 2006 Sep 7.
8
One-Pot Synthesis of Hyperoside by a Three-Enzyme Cascade Using a UDP-Galactose Regeneration System.利用UDP-半乳糖再生系统通过三酶级联一锅法合成金丝桃苷
J Agric Food Chem. 2017 Jul 26;65(29):6042-6048. doi: 10.1021/acs.jafc.7b02320. Epub 2017 Jul 10.
9
Orotate decreases the inhibitory effect of ethanol on galactose elimination in the perfused rat liver.乳清酸可降低乙醇对灌流大鼠肝脏中半乳糖清除的抑制作用。
Biochem J. 1976 Dec 15;160(3):715-20. doi: 10.1042/bj1600715.
10
Changing activities of galactose-metabolizing enzymes during perfusion of suckling-rat liver.乳鼠肝脏灌注过程中半乳糖代谢酶活性的变化
Am J Physiol. 1981 Mar;240(3):E333-9. doi: 10.1152/ajpendo.1981.240.3.E333.
引用本文的文献
1
Metabolism of galactose in the brain and liver of rats and its conversion into glutamate and other amino acids.大鼠脑和肝脏中半乳糖的代谢及其向谷氨酸和其他氨基酸的转化。
J Neural Transm (Vienna). 2009 Feb;116(2):131-9. doi: 10.1007/s00702-008-0166-9. Epub 2008 Dec 17.
2
Sulphated glycosaminoglycans in regenerating rat liver.再生大鼠肝脏中的硫酸化糖胺聚糖
Biochem J. 1980 Jun 15;188(3):769-73. doi: 10.1042/bj1880769.
3
Different turnover of fucose residues in plasma membranes of rat liver and Morris hepatoma 7777.大鼠肝脏和莫里斯肝癌7777细胞膜中岩藻糖残基的不同周转率。
Biochem J. 1980 Jul 15;190(1):51-5. doi: 10.1042/bj1900051.
4
Does the D-galactose receptor on Kupffer cells recycle?库普弗细胞上的D-半乳糖受体是否会循环利用？
Biochem J. 1981 Nov 15;200(2):445-8. doi: 10.1042/bj2000445.
5
Human lysosomes can be purified from diploid skin fibroblasts by free-flow electrophoresis.人类溶酶体可以通过自由流动电泳从二倍体皮肤成纤维细胞中纯化出来。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6139-43. doi: 10.1073/pnas.77.10.6139.
6
Increased glycosylation capacity in regenerating rat liver is paralleled by decreased activities of CMP-N-acetylneuraminate hydrolase and UDP-galactose pyrophosphatase.再生大鼠肝脏中糖基化能力的增强与CMP-N-乙酰神经氨酸水解酶和UDP-半乳糖焦磷酸酶活性的降低同时出现。
Biochem J. 1983 Sep 15;214(3):1003-6. doi: 10.1042/bj2141003.
7
Glucosamine metabolism in regenerating rat liver.再生大鼠肝脏中的葡萄糖胺代谢
Biochem J. 1976 Sep 15;158(3):589-92. doi: 10.1042/bj1580589.
8
Sialyltransferase activity in regenerating rat liver.再生大鼠肝脏中的唾液酸转移酶活性
Biochem J. 1977 Sep 15;166(3):381-6. doi: 10.1042/bj1660381.
9
Subcellular localization of the enzyme that forms mannosyl retinyl phosphate from guanosine diphosphate [14C]mannose and retinyl phosphate.由二磷酸鸟苷[14C]甘露糖和视黄基磷酸形成甘露糖基视黄基磷酸的酶的亚细胞定位。
Biochem J. 1979 Jun 15;180(3):449-53. doi: 10.1042/bj1800449a.
10
Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy.[5-³H]尿苷的合成代谢与分解代谢及其与部分肝切除术后小鼠肝脏核糖核酸标记的关系
Biochem J. 1979 Jan 15;178(1):1-8. doi: 10.1042/bj1780001.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
MAMMALIAN GALACTOKINASE. DEVELOPMENTAL AND ADAPTIVE CHARACTERISTICS IN THE RAT LIVER.哺乳动物半乳糖激酶。大鼠肝脏中的发育及适应性特征
J Biol Chem. 1965 Jun;240:2382-8.
3
A PARADOXICAL EFFECT OF ACTINOMYCIN D: THE MECHANISM OF REGULATION OF ENZYME SYNTHESIS BY HYDROCORTISONE.放线菌素D的一种矛盾效应：氢化可的松对酶合成的调节机制
Proc Natl Acad Sci U S A. 1964 Oct;52(4):1121-9. doi: 10.1073/pnas.52.4.1121.
4
PERIODATE OXIDATION OF THE GLYCOPROTEIN FETUIN.糖蛋白胎球蛋白的高碘酸盐氧化作用。
J Biol Chem. 1964 Feb;239:567-73.
5
Rapid enzyme assay technique utilizing radioactive substrate, ion-exchange paper, and liquid scintillation counting.利用放射性底物、离子交换纸和液体闪烁计数的快速酶测定技术。
Anal Biochem. 1963 Jun;5:548-54. doi: 10.1016/0003-2697(63)90075-7.
6
7
Oligosaccharides from the alpha1 acid glycoprotein of human plasma.人血浆α1酸性糖蛋白中的寡糖
J Biol Chem. 1962 Mar;237:622-8.
8
[A simple technic for extremely rapid freezing of large pieces of tissue].[一种用于极快速冷冻大块组织的简单技术]
Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:399-412.
9
A convenient, rapid and sensitive method for measuring the incorporation of radioactive amino acids into protein.一种用于测量放射性氨基酸掺入蛋白质的简便、快速且灵敏的方法。
Biochem Biophys Res Commun. 1960 Nov;3:540-3. doi: 10.1016/0006-291x(60)90171-6.
10
The enzymic interconversion of uridine diphosphogalactose and uridine diphosphoglucose.尿苷二磷酸半乳糖与尿苷二磷酸葡萄糖的酶促相互转化。
J Biol Chem. 1957 Nov;229(1):139-51.
Zotero 插件