用于查加斯病的微孔板酶联免疫吸附测定
Microplate enzyme-linked immunosorbent assay for chagas' disease.
作者信息
Voller A, Draper C, Bidwell D E, Bartlett A
出版信息
Lancet. 1975 Feb 22;1(7904):426-8. doi: 10.1016/s0140-6736(75)91492-0.
A microscale immunosorbent assay (E.L.I.S.A.) for the measurement of antibody in Chagas' disease (American trypanosomiasis) is described. The test was assessed on plasma samples from people living in Bahia, Brazil. 98% of the individuals with positive Trypanosoma cruzi immunofluorescent-antibody (I.F.A.) tests gave E.L.I.S.A. reading considered as positive. Less than 2% of those with negative I.F.A. tests had positive E.L.I.S.A. reactions. This simple test may be suitable for use in large seroepidemiological surveys.
本文描述了一种用于检测恰加斯病（美洲锥虫病）抗体的微量免疫吸附测定法（酶联免疫吸附测定，ELISA）。该检测方法在来自巴西巴伊亚州居民的血浆样本上进行了评估。克鲁斯锥虫免疫荧光抗体（IFA）检测呈阳性的个体中，98%的ELISA检测结果也被视为阳性。IFA检测呈阴性的个体中，不到2%的人ELISA反应呈阳性。这种简单的检测方法可能适用于大规模血清流行病学调查。
Zotero 插件