Hoshino-Shimizu S, Camargo M E, Umezawa E S
Am J Trop Med Hyg. 1975 Jul;24(4):586-9. doi: 10.4269/ajtmh.1975.24.586.
A slide flocculation test for Chagas' disease is described, which uses a lyophilized, stable antigen obtained by formalin and ultrasonic treatment of culture forms of Trypanosoma cruzi. The test was compared with other tests for the serodiagnosis of American trypanosomiasis and showed a high sensitivity, positive results being obtained in every case of acute or chronic Chagas' disease. In sera from blood donors and from normal individuals with negative T. cruzi antigen complement fixation tests a specificity of 96% was found. False positive flocculation tests were seen, especially in cases of South American blastomycosis and in a few cases of acute toxoplasmosis. Since it is easy and quick to perform, the slide flocculation test can be recommended as a screening procedure, especially for blood banks.
本文描述了一种用于查加斯病的玻片凝集试验，该试验使用经福尔马林和超声处理的克氏锥虫培养物形式获得的冻干稳定抗原。将该试验与其他美洲锥虫病血清学诊断试验进行了比较，结果显示其具有高敏感性，在急性或慢性查加斯病的每一病例中均获得阳性结果。在献血者血清以及克氏锥虫抗原补体结合试验阴性的正常个体血清中，发现该试验的特异性为96%。出现了假阳性凝集试验结果，尤其是在南美芽生菌病病例中，以及少数急性弓形虫病病例中。由于该玻片凝集试验操作简便快捷，可推荐作为一种筛查方法，特别是用于血库。
