Pratten M K, Lloyd J B
Biochem J. 1979 Jun 15;180(3):567-71. doi: 10.1042/bj1800567.
Low temperature, NaF and 2,4-dinitrophenol could each abolish the pinocytic uptake of 125I-labelled poly(vinylpyrrolidone) or colloidal [198Au]gold by rat peritoneal macrophages cultured in vitro. Cytochalasin B caused only partial inhibition, even at 10 microgram/ml, and colchicine (10 or 25 microgram/ml) inhibited uptake of colloidal [198Au]gold much more than that of 125I-labelled poly(vinylpyrrolidone). Dibutyryl cyclic AMP and ouabain were without effect on uptake of 125I-labelled poly(vinylpyrrolidone), and slight stimulation was seen with ATP and theophylline. Uptake of 125I-labelled poly(vinylpyrrolidone) was abolished by EGTA (5mM), but restored by adding CaCl2 (5mM). The results appear not to support the conventional criteria for the division of pinocytic phenomena into macropinocytosis, requiring a metabolic energy supply and cytoskeletal components, and micropinocytosis, requiring neither.
低温、氟化钠和2,4 -二硝基苯酚均可消除体外培养的大鼠腹腔巨噬细胞对125I标记的聚乙烯吡咯烷酮或胶体[198Au]金的胞饮摄取。细胞松弛素B即使在10微克/毫升时也仅引起部分抑制,而秋水仙碱(10或25微克/毫升)对胶体[198Au]金摄取的抑制作用远大于对125I标记的聚乙烯吡咯烷酮摄取的抑制作用。二丁酰环磷腺苷和哇巴因对125I标记的聚乙烯吡咯烷酮的摄取没有影响,而ATP和茶碱则有轻微的刺激作用。5毫摩尔的乙二醇双四乙酸(EGTA)可消除125I标记的聚乙烯吡咯烷酮的摄取,但添加5毫摩尔的氯化钙可使其恢复。结果似乎不支持将胞饮现象分为巨胞饮作用(需要代谢能量供应和细胞骨架成分)和微胞饮作用(两者均不需要)的传统标准。