Duncan R, Lloyd J B
Biochim Biophys Acta. 1978 Dec 18;544(3):647-55. doi: 10.1016/0304-4165(78)90339-2.
Low temperature,2,4-dinitrophenol and moniodoacetate could each completely abolish the pinocytic uptake of 125I-labelled polyvinylpyrrolidone, 125I-labelled bovine serum albumin or colloidal 198 Au by 17.5-day rat visceral yolk sac cultured in vitro. Cytochalasin B and colchicine caused a partial and dose-dependent inhibition. It is concluded that the mechanism of pinocytic uptake of these substrates is not micropinocytosis as conventionally defined. Removal of extracellular calcium or the presence of theophylline inhibited liquid-phase pinocytosis by the rat yolk sac, whereas addition of ouabain caused a biphasic response: a slight stimulation of pinosome formation at a low concentration, and an inhibitory effect at a higher concentration.
低温、2,4 -二硝基苯酚和碘乙酸均可完全抑制体外培养的17.5天龄大鼠内脏卵黄囊对125I标记的聚乙烯吡咯烷酮、125I标记的牛血清白蛋白或胶体198Au的胞饮摄取。细胞松弛素B和秋水仙碱引起部分且呈剂量依赖性的抑制作用。由此得出结论，这些底物的胞饮摄取机制并非传统定义的微胞饮作用。去除细胞外钙或存在茶碱会抑制大鼠卵黄囊的液相胞饮作用，而加入哇巴因会引起双相反应：低浓度时对胞饮体形成有轻微刺激作用，高浓度时则有抑制作用。
