大鼠腹膜巨噬细胞中胞饮作用和细胞内蛋白水解的定量研究。
A quantitative study of pinocytosis and intracellular proteolysis in rat peritoneal macrophages.
作者信息
Pratten M K, Williams K E, Lloyd J B
出版信息
Biochem J. 1977 Dec 15;168(3):365-72. doi: 10.1042/bj1680365.
A method for the culture of rat peritoneal macrophages in vitro is described, in which pinocytic uptake of colloidal [198 Au]gold, 125I--labelled poly(vinylpyrrolidone) and [14C]sucrose proceeds at contant and fairly reproducible rates for several hours. The rat of uptake of colloidal [198 Au]gold, which wxhibited some inter-batch variation, was approx. 100 times that of the other two substrates. Colloidal gold did not affect the rate of uptake of 125I-labelled poly(vinylpyrrolidone) and therefore its own high rate of uptake could not be attributed to a stimulation of the formation of pinocytic vesicles. It conclude that uptake of collodial gold is highly dependent on adsorption on binding sites on the plasma membrane. Uptake of formaldehyde-treated 125I-labelled bovine serum albumin was followed by the release of [125I]iodo-L-tyrosine into the culture medium and took place at a rate intermediate between those of collodial [198Au]gold and the other two non-digestible substrates, 125I-labelled poly(vinylpyrrolidone) and [14C]sucrose.
本文描述了一种大鼠腹膜巨噬细胞的体外培养方法，在此方法中，胶体[198Au]金、125I标记的聚乙烯吡咯烷酮和[14C]蔗糖的胞饮摄取在数小时内以恒定且相当可重复的速率进行。胶体[198Au]金的摄取率表现出一定的批次间差异，约为其他两种底物摄取率的100倍。胶体金不影响125I标记的聚乙烯吡咯烷酮的摄取速率，因此其自身的高摄取率不能归因于对胞饮小泡形成的刺激。得出的结论是，胶体金的摄取高度依赖于其在质膜结合位点上的吸附。甲醛处理的125I标记牛血清白蛋白的摄取之后，[125I]碘-L-酪氨酸释放到培养基中，其摄取速率介于胶体[198Au]金与其他两种不可消化底物（125I标记的聚乙烯吡咯烷酮和[14C]蔗糖）的摄取速率之间。
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