使用稳态速率方程分析进程曲线数据。

The use of steady-state rate equations to analyse progress curve data.

作者信息

Duggleby R G, Morrison J F

出版信息

Biochim Biophys Acta. 1979 Jun 6;568(2):357-62. doi: 10.1016/0005-2744(79)90303-6.

Abstract

Analysis of progress curves for enzyme-catalyzed reactions has been made by using a procedure that does not require the derivation of complex integrated rate equations. The method involves conversion of progress curve data to reaction velocities that are then fitted to the appropriate differential rate equation. Application of the procedure to data obtained for the reaction catalyzed by aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1), showed that the resulting values for the kinetic parameters agreed well with those obtained by conventional progress curve analysis (Duggleby, R.G. and Morrison, J.F. (1978) Biochim. Biophys. Acta 526, 398--409).

摘要

通过使用一种无需推导复杂积分速率方程的程序，对酶催化反应的进程曲线进行了分析。该方法包括将进程曲线数据转换为反应速度，然后将其拟合到适当的微分速率方程中。将该程序应用于天冬氨酸转氨酶（L-天冬氨酸：2-氧代戊二酸转氨酶，EC 2.6.1.1）催化反应所获得的数据，结果表明，动力学参数的所得值与通过传统进程曲线分析获得的值非常吻合（Duggleby, R.G. 和 Morrison, J.F. (1978) Biochim. Biophys. Acta 526, 398 - 409）。