污水和活性污泥中的需氧异养细菌群体。II. 活性污泥细菌的表征方法。
Aerobic heterotrophic bacterial populations of sewage and activated sludge. II. Method of characterization of activated sludge bacteria.
作者信息
Prakasam T B, Dondero N C
出版信息
Appl Microbiol. 1967 Sep;15(5):1122-7. doi: 10.1128/am.15.5.1122-1127.1967.
Abstract
The replica-plating technique and Lochhead's nutritional method were combined in exploratory experiments to test their feasibility as useful means for characterizing the aerobic heterotrophic flora of activated sludge and to minimize the burdensome process of isolation, purification, and testing of isolates. In the test run, the method was about 86% reliable at the 0.05 level of significance. About 40% of the total number of bacteria able to grow on an aqueous extract of activated sludge did not grow on media containing glucose, amino acids, growth factors, and inorganic salts. The requirement for activated sludge extract suggested the existence of a requirement for unidentified nutrients contained in the activated sludge extract.
摘要
在探索性实验中，将影印平板技术与洛赫黑德营养法相结合，以测试它们作为表征活性污泥需氧异养菌群的有用手段的可行性，并尽量减少分离、纯化和测试分离物这一繁琐过程。在试运行中，该方法在0.05显著性水平下的可靠性约为86%。能够在活性污泥水提取物上生长的细菌总数中，约40%在含有葡萄糖、氨基酸、生长因子和无机盐的培养基上不能生长。对活性污泥提取物的需求表明，活性污泥提取物中存在对未鉴定营养物质的需求。
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