污水和活性污泥中的需氧异养细菌种群。I. 计数
Aerobic heterotrophic bacterial populations of sewage and activated sludge. I. Enumeration.
作者信息
Prakasam T B, Dondero N C
出版信息
Appl Microbiol. 1967 May;15(3):461-7. doi: 10.1128/am.15.3.461-467.1967.
Abstract
Agar plating media containing solely activated sludge extracts yielded, in general, higher viable counts of activated sludge bacteria than any other culture medium tested. Activated sludge extracts made from different treatment plants varied in efficacy in evoking maximal viable counts. Frequently, homologous plating, i.e., plating inocula of activated sludges on extracts made from the same activated sludges, tended to yield lower counts than the heterologous platings tried in this investigation. The counts obtained by homologous plating of activated sludge were not significantly lower and sometimes were even significantly higher than the counts obtained on standard Nutrient Agar, which had been found by previous workers to be a good medium for counting activated sludge bacteria. The higher counts obtained with activated sludge extracts set objectives for formulating reproducible or defined culture media for the enumeration of activated sludge bacteria.
摘要
一般来说，仅含有活性污泥提取物的琼脂平板培养基所培养出的活性污泥细菌活菌数，要高于所测试的任何其他培养基。不同处理厂制备的活性污泥提取物在激发最大活菌数方面的效果有所不同。在本研究中，同源平板接种法，即将活性污泥接种于由相同活性污泥制备的提取物上，其活菌数往往低于异源平板接种法。活性污泥同源平板接种法所获得的活菌数并不显著低于在标准营养琼脂上获得的活菌数，有时甚至显著高于该活菌数，而先前的研究人员已发现标准营养琼脂是用于计数活性污泥细菌的良好培养基。活性污泥提取物所获得的较高活菌数为配制用于活性污泥细菌计数的可重现或限定培养基设定了目标。
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