Zacharias B, Björklund M
Appl Microbiol. 1968 Jan;16(1):69-72. doi: 10.1128/am.16.1.69-72.1968.
The continuous production of Clostridium tetani toxin has been carried out in a 1-liter stirred culture vessel for as long as 65 days. Toxin production of approximately 120 flocculating units per ml was maintained with a dilution rate of 0.125 hr(-1), a temperature of 34 C, a pH of 7.4, and the addition to the medium of 0.1 g of potassium chloride per liter. The average minimal lethal intraperitoneal dose of the toxin in mice was approximately 10(6) per ml.
破伤风梭菌毒素的连续生产已在1升搅拌培养容器中持续进行了长达65天。在稀释率为0.125小时^(-1)、温度为34℃、pH值为7.4且每升培养基添加0.1克氯化钾的条件下，维持了约每毫升120个絮凝单位的毒素产量。该毒素对小鼠的平均最小腹腔致死剂量约为每毫升10^6。
