Brian B L, Gardner E W
Appl Microbiol. 1968 Apr;16(4):549-52. doi: 10.1128/am.16.4.549-552.1968.
Four gram-negative bacterial species, including Escherichia coli strain B, Serratia marcescens, Pseudomonas fluorescens, and Vibrio cholerae (comma) strain NIH 41, were investigated for fatty acid content by gas-liquid chromatography involving a preparatory technique which facilitated detection of cyclopropane fatty acids. Methyl esters of fatty acids were subjected to mild catalytic hydrogenation to eliminate unsaturates. Hydrogenation was followed by bromination which removed cyclopropane acids from chromatographic profile patterns. Lactobacillic acid (cis-11,12-methyleneoctanoate) and cis-9,10-methylenehexadecanoate, previously reported in lipids of E. coli and S. marcescens, were found in small amounts in P. fluorescens but were not detected in V. cholerae.
通过气相色谱法对包括大肠杆菌B菌株、粘质沙雷氏菌、荧光假单胞菌和霍乱弧菌（逗号）NIH 41菌株在内的四种革兰氏阴性细菌进行了脂肪酸含量研究，该气相色谱法采用了一种有助于检测环丙烷脂肪酸的制备技术。脂肪酸甲酯经过温和的催化氢化以消除不饱和物。氢化之后进行溴化，从而从色谱图谱中去除环丙烷酸。先前在大肠杆菌和粘质沙雷氏菌的脂质中报道过的乳杆菌酸（顺式-11,12-亚甲基辛酸酯）和顺式-9,10-亚甲基十六烷酸酯在荧光假单胞菌中含量较少，但在霍乱弧菌中未检测到。
