Dees S B, Hollis D G, Weaver R E, Moss C W
J Clin Microbiol. 1983 Nov;18(5):1073-8. doi: 10.1128/jcm.18.5.1073-1078.1983.
The cellular fatty acid compositions of the lectotype strain and four clinical isolates of Pseudomonas marginata were determined by gas-liquid chromatography and compared with 11 strains of the Centers for Disease Control Pseudomonas-like group 2, which are similar to P. marginata in a number of conventional biochemical tests. Isolates of P. marginata were readily distinguished from Pseudomonas-like group 2 by the presence of a C17:0 cyclopropane acid and hydroxy acids 3-OH-C14:0, 2-OH-C16:0, 3-OH-C16:0, and 2-OH-C18:1, whereas strains of Pseudomonas-like group 2 contained C16:1 delta 9 as a major acid with small amounts of 3-OH-C12:0 and 2-OH-C14:0 acids. Our data show that cellular fatty acid composition provides useful additional information that can be combined with selected conventional tests to provide a more reliable and rapid identification of P. marginata and related bacteria.
通过气液色谱法测定了边缘假单胞菌模式菌株和四株临床分离株的细胞脂肪酸组成，并与疾病控制中心假单胞菌样菌群2的11株菌株进行了比较，这11株菌株在一些传统生化试验中与边缘假单胞菌相似。边缘假单胞菌分离株通过存在C17:0环丙烷酸和羟基酸3-OH-C14:0、2-OH-C16:0、3-OH-C16:0和2-OH-C18:1，很容易与假单胞菌样菌群2区分开来，而假单胞菌样菌群2的菌株以C16:1Δ9作为主要酸，含有少量的3-OH-C12:0和2-OH-C14:0酸。我们的数据表明，细胞脂肪酸组成提供了有用的额外信息，可与选定的传统试验相结合，以更可靠、快速地鉴定边缘假单胞菌及相关细菌。
