Blaser M J, Moss C W, Weaver R E
J Clin Microbiol. 1980 May;11(5):448-51. doi: 10.1128/jcm.11.5.448-451.1980.
The cellular fatty acid composition of 29 human and animal isolates of Campylobacter fetus was determined with gas-liquid chromatography. All strains contained small amounts of 3-hydroxytetradecanoic acid, suggesting the presence of lipid A. Each of 13 different blood or stool isolates of C. fetus subsp. jejuni obtained from humans or fowl contained a 19-carbon cyclopropane acid which was not present in C. fetus subsp. fetus (6 strains from cattle) or C. fetus subsp. intestinalis (10 strains from humans and cattle). These findings indicate that C. fetus subsp. jejuni can be rapidly differentiated from other C. fetus by gas-liquid chromatography analysis of cellular fatty acids.
采用气液色谱法测定了29株人及动物胎儿弯曲菌分离株的细胞脂肪酸组成。所有菌株均含有少量的3-羟基十四烷酸,提示存在脂多糖。从人或禽类中获得的13株不同的胎儿弯曲菌空肠亚种血液或粪便分离株,每株都含有一种19碳环丙烷酸,而在胎儿弯曲菌胎儿亚种(6株来自牛)或胎儿弯曲菌肠道亚种(10株来自人和牛)中不存在。这些结果表明,通过细胞脂肪酸的气液色谱分析,胎儿弯曲菌空肠亚种可迅速与其他胎儿弯曲菌区分开来。