Gazendam J, Go K G, van Zanten A K
Brain Res. 1979 Oct 19;175(2):279-90. doi: 10.1016/0006-8993(79)91007-2.
Brain edema fluid was collected from cats with a freezing lesion in the left parietal cortex by the insertion into the brain of needles containing nylon wicks and connected to polyethylene tubes. The edema fluid samples which accumulated in the polyethylene tubes were regularly analyzed for Na+ and K+ content, colloid osmotic pressure, lactate dehydrogenase and creatine phosphokinase activity, and 99mTc-albumin radioactivity; the albumin tracer being introduced intravenously at the time of cold-injury. One series of cats received an intracerebral injection of ouabain solution, the control series an intracerebral injection of saline, at 100 min after the cold-injury. The ouabain injection was followed by an increase of K+ content, LDH and CPK activities but a decrease of Na+ concentration in the edema fluid, attributable to a concentration of solutes in the edema fluid as presumably water and Na+ were shifted into the cells and hence the extracellular space was reduced.
通过将装有尼龙灯芯并与聚乙烯管相连的针插入大脑，从左顶叶皮质有冷冻损伤的猫身上收集脑水肿液。定期分析积聚在聚乙烯管中的水肿液样本的钠和钾含量、胶体渗透压、乳酸脱氢酶和肌酸磷酸激酶活性，以及99m锝 - 白蛋白放射性；在冷损伤时静脉注射白蛋白示踪剂。一组猫在冷损伤后100分钟接受脑内注射哇巴因溶液，对照组接受脑内注射生理盐水。注射哇巴因后，水肿液中的钾含量、乳酸脱氢酶和肌酸磷酸激酶活性增加，但钠浓度降低，这可能是由于水肿液中的溶质浓缩，因为水和钠可能转移到细胞中，从而细胞外间隙减小。
