Kenny C P, Ashton F E, Diena B B, Greenberg L
Bull World Health Organ. 1967;37(4):569-73.
Chemically defined, protein-free, liquid culture media are needed for research with pathogenic bacteria and for the preparation of vaccines from them. Variations in growth or yield of organisms should be due only to differences in strain, and the medium itself should have little or no antigenic properties or toxicity. The medium should maintain the integrity of the bacterial species fully and it is particularly important that the complete antigenic complement of the organism should remain unaltered. The medium should be inexpensive and simple to prepare. Such a medium for the cultivation of some species of Neisseria, known to be fastidious, is described.In this new medium all the criteria are satisfied, and its freedom from sensitizing proteins makes it of particular value for use in man. Results of safety tests of vaccines prepared from Neisseria gonorrhoeae and N. meningitidis grown in this medium were encouraging. It should also be useful in studies on the physiological characteristics of the individual species.
研究致病细菌以及利用它们制备疫苗需要化学成分明确、不含蛋白质的液体培养基。生物体生长或产量的差异应仅归因于菌株差异，并且培养基本身应几乎没有或没有抗原特性或毒性。培养基应能充分维持细菌物种的完整性，特别重要的是生物体的完整抗原补体应保持不变。培养基应价格低廉且制备简单。本文描述了一种用于培养某些已知苛求的奈瑟菌属物种的培养基。在这种新培养基中，所有标准都得到了满足，并且其不含致敏蛋白使其在人体应用中具有特殊价值。用在此培养基中生长的淋病奈瑟菌和脑膜炎奈瑟菌制备的疫苗的安全性测试结果令人鼓舞。它在对各个物种的生理特性研究中也应会有用。
