用戊二醛和四氧化锇同时固定，然后在醋酸铀中“后固定”后人白细胞的超微结构
Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and "postfixation" in uranyl acetate.
作者信息
Hirsch J G, Fedorko M E
出版信息
J Cell Biol. 1968 Sep;38(3):615-27. doi: 10.1083/jcb.38.3.615.
Abstract
Human leukocytes in suspension or in monolayer cultures have been processed for electron microscopy by fixation in a freshly made cold mixture of glutaraldehyde and osmium tetroxide and by "postfixation" in uranyl acetate. Simultaneous exposure to glutaraldehyde and osmium tetroxide eliminates many of the shortcomings seen when either of these agents is used alone as the initial fixative. Specimens are processed to the stage of dehydration as single cell suspensions or as very small clumps to assure rapid penetration of fixatives and efficient washing. The technique is rapid and reproducible. Electron micrographs presented in this report illustrate the ultrastructural features of human white cells prepared by this method.
摘要
悬浮或单层培养的人白细胞已通过以下方法处理用于电子显微镜观察
用新鲜配制的冷戊二醛和四氧化锇混合物固定，并在醋酸铀中进行“后固定”。同时暴露于戊二醛和四氧化锇可消除单独使用这两种试剂作为初始固定剂时出现的许多缺点。将标本作为单细胞悬液或非常小的团块处理至脱水阶段，以确保固定剂快速渗透和有效洗涤。该技术快速且可重复。本报告中的电子显微照片展示了用该方法制备的人白细胞的超微结构特征。
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本文引用的文献
1
A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.一种用于电子显微镜的简化柠檬酸铅染色法。
J Cell Biol. 1965 May;25(2):407-8. doi: 10.1083/jcb.25.2.407.
2
ELECTRON MICROSCOPE STUDIES ON THE DEGRANULATION OF RABBIT PERITONEAL LEUKOCYTES DURING PHAGOCYTOSIS.吞噬作用期间兔腹膜白细胞脱颗粒的电子显微镜研究
J Exp Med. 1964 Oct 1;120(4):569-76. doi: 10.1084/jem.120.4.569.
3
GOLGI APPARATUS AND LYSOSOMES.高尔基体和溶酶体。
Fed Proc. 1964 Sep-Oct;23:1010-22.
4
FIBRILLAR STRUCTURES IN THE CELLS OF BLOODFORMING ORGANS.造血器官细胞中的纤维状结构。
J Natl Cancer Inst. 1964 Sep;33:467-85.
5
6
7
THE ULTRASTRUCTURE OF CELLS IN HUMAN THORACIC DUCT LYMPH.人胸导管淋巴中细胞的超微结构
J Ultrastruct Res. 1963 Oct;59:325-39. doi: 10.1016/s0022-5320(63)80010-6.
8
Filamentous structures in the cytoplasm of normal mononuclear phagocytes.正常单核吞噬细胞胞质中的丝状结构。
J Ultrastruct Res. 1962 Aug;7:39-55. doi: 10.1016/s0022-5320(62)80025-2.
9
Electron microscopy of human white blood cells and their stem cells.人类白细胞及其干细胞的电子显微镜检查。
Int Rev Cytol. 1961;12:199-241. doi: 10.1016/s0074-7696(08)60541-0.
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