Walsh P R, Abarca S D, Wang M C
Clin Biochem. 1979 Aug;12(4):118-22. doi: 10.1016/s0009-9120(79)80136-8.
The solid-phase radioimmunoassay procedures for serum digoxin and cortisol with the Concept-4 were evaluated for specificity, correlation with other radioimmunoassay procedures, parallelism, recovery, carryover, and precision. 1. The detection limits of the digoxin and cortisol assays were 10 pg/tube and 40 pg/tube, respectively. These detections correspond to serum concentrations of 0.25 micrograms/liter and 20 micrograms/liter, respectively, for digoxin and cortisol. 2. Specificity of the digoxin assay was characterized by a 18.3% cross reaction with digitoxin. The anti-cortisol with 11-deoxycortisol, cross reaction with 11-deoxycortisol. 3. The values obtained with the Concept-4 for both procedures demonstrated acceptable correlations with other existing RIA procedures. 4. Dilution studies conducted to determine the parallelism of the 2 assays indicated that both assays demonstrated excellent proportionality between the volume of specimen and the quantity of analyte measured. 5. Analytical recoveries of digoxin and cortisol added to the individual assays average 95% and 101%, respectively. 6. Carryover studies indicated a routine sample-to-sample interaction of less than 0.05% for both assays. 7. The digoxin assay routinely demonstrated intra- and inter-assay variations of less than 7.4% and less than 10.0%, respectively. The precision data for cortisol indicated intra- and inter-assay variations of less than 6.7% and less than 9.1%, respectively.
对使用Concept-4进行血清地高辛和皮质醇的固相放射免疫分析程序的特异性、与其他放射免疫分析程序的相关性、平行性、回收率、携带污染和精密度进行了评估。1. 地高辛和皮质醇检测的检测限分别为10 pg/管和40 pg/管。这些检测限分别对应地高辛和皮质醇的血清浓度为0.25微克/升和20微克/升。2. 地高辛检测的特异性表现为与洋地黄毒苷有18.3%的交叉反应。抗皮质醇与11-脱氧皮质醇有交叉反应。3. 使用Concept-4获得的两种检测程序的值与其他现有的放射免疫分析程序显示出可接受的相关性。4. 为确定两种检测的平行性而进行的稀释研究表明，两种检测在标本体积和所测分析物数量之间均表现出良好的比例关系。5. 加入到各自检测中的地高辛和皮质醇的分析回收率平均分别为95%和101%。6. 携带污染研究表明，两种检测的常规样品间相互作用均小于0.05%。7. 地高辛检测常规显示批内和批间变异分别小于7.4%和小于10.0%。皮质醇的精密度数据表明批内和批间变异分别小于6.7%和小于9.1%。
