Valdes R, Wills M R, Savory J
Ann Clin Lab Sci. 1980 Nov-Dec;10(6):508-14.
The performance characteristics are reported for a serum cortisol assay using the GAMMAFLO Automated Assay System, an automated continuous-flow system for radioimmunoassay (RIA). This automated system utilizes a combination of continuous-flow methodology and column chromatography techniques. The cortisol assay studied had a standard curve range of 10.0 to 500 microgram per L. The intra-assay and inter-assay precision were 5.1 percent and 7.2 percent (RSD), respectively, for a cortisol concentration of 160 microgram per L. The automated serum cortisol results correlated (r = 0.958 and 0.933) with two established manual radioimmunoassay procedures. No drift or carryover was detectable in 200 within-assay consecutive determinations performed at a rate of 42 determinations per hour (five hours continuous operation). The automated system studied appears to offer a viable alternative to cortisol manual RIA techniques in terms of overall precision simplicity of operation, and sample throughput.
本文报道了使用GAMMAFLO自动分析系统检测血清皮质醇的性能特征，该系统是一种用于放射免疫分析（RIA）的自动连续流动系统。这个自动化系统结合了连续流动方法和柱色谱技术。所研究的皮质醇检测方法的标准曲线范围为每升10.0至500微克。对于每升160微克的皮质醇浓度，批内精密度和批间精密度分别为5.1%和7.2%（相对标准偏差）。自动化血清皮质醇检测结果与两种既定的手动放射免疫分析方法具有相关性（r = 0.958和0.933）。在以每小时42次检测的速率（连续运行5小时）进行的200次批内连续检测中，未检测到漂移或残留。就总体精密度、操作简便性和样品通量而言，所研究的自动化系统似乎为皮质醇手动放射免疫分析技术提供了一种可行的替代方法。
