一种用于测定组织匀浆血红蛋白含量的简单特异方法。
A simple specific method for the determination of the hemoglobin content of tissue homogenates.
作者信息
Marklund S
出版信息
Clin Chim Acta. 1979 Mar 1;92(2):229-34. doi: 10.1016/0009-8981(79)90117-7.
PMID:487574
Abstract
A simple, inexpensive and specific method for the determination of hemoglobin in tissue homogenates is described. It is based on the specific binding of hemoglobin by haptoglobin and the stabilizing effect of this binding on the peroxidase activity of hemoglobin. The haptoglobin is added as serum and no purification is needed. The method gives reliable estimates of hemoglobin contents above 10 mg/l.
摘要
本文描述了一种用于测定组织匀浆中血红蛋白的简单、廉价且特异的方法。该方法基于触珠蛋白与血红蛋白的特异性结合以及这种结合对血红蛋白过氧化物酶活性的稳定作用。触珠蛋白以血清形式添加，无需纯化。该方法能可靠地估计血红蛋白含量高于10mg/l的情况。
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