DNA的剪切与熔解：大肠杆菌基因组中一个特别敏感的部分。
Shear and the melting of DNA: an especially sensitive portion of the E. coli genome.
作者信息
Rosenberg B H, Cavalieri L F
出版信息
Biophys J. 1968 Oct;8(10):1138-45. doi: 10.1016/s0006-3495(68)86545-2.
The melting point of DNA is shown to be a function of shear stress. The higher the molecular weight of the DNA, the further its melting point is lowered by a given shear rate. During lysis of E. coli, a part of the DNA is especially shear sensitive, so that its melting curve in the presence of shear shows a low-melting region prior to the main transition. Lysis and dilution of the cell contents destroys the extra shear sensitivity, perhaps because the DNA dissociates from the cell membrane or from some other large subcellular structure. Such a structure would impart increased shear sensitivity to the associated region of the genome.
DNA的熔点被证明是剪切应力的函数。DNA的分子量越高，在给定剪切速率下其熔点降低得就越多。在大肠杆菌裂解过程中，一部分DNA对剪切特别敏感，因此在有剪切的情况下其熔解曲线在主要转变之前会出现一个低熔点区域。细胞内容物的裂解和稀释消除了额外的剪切敏感性，这可能是因为DNA从细胞膜或其他一些大的亚细胞结构上解离下来。这样的结构会使基因组的相关区域对剪切的敏感性增加。
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