小牛胸腺组蛋白自聚集的碳磁共振研究。
Carbon magnetic resonance studies of the self-aggregation of calf thymus histones.
作者信息
Tancredi T, Temussi P A, Di Pascale G, Fournier C
出版信息
Eur J Biochem. 1979 Oct;100(1):219-24. doi: 10.1111/j.1432-1033.1979.tb02052.x.
Self-aggregation of calf thymus histones H2A and H4 was studied by means of 13C magnetic resonance. Analyses of the changes in the intensities of several side-chain resonances, induced by added NaCl, confirm that carbon magnetic resonance can be used to monitor aggregation phenomena more accurately than other physicochemical methods. In particular the validity of an original computer-based method to treat 13C intensities is confirmed. The details of these aggregation phenomena are critically discussed in the light of a simple experiment on a 13C-enriched sample of H2B.
通过碳-13磁共振研究了小牛胸腺组蛋白H2A和H4的自聚集。对添加氯化钠引起的几个侧链共振强度变化的分析证实，与其他物理化学方法相比，碳磁共振可更准确地用于监测聚集现象。特别是，一种基于计算机处理碳-13强度的原始方法的有效性得到了证实。根据对富含碳-13的H2B样品进行的一项简单实验，对这些聚集现象的细节进行了批判性讨论。
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