一项使用碳-13磁共振技术对小牛胸腺组蛋白H2B进行的研究。

A study of calf-thymus histone H2B using 13C magnetic resonance.

作者信息

Tancredi T, Temussi P A, Paolillo L, Trivellone E, Crane-Robinson C

出版信息

Eur J Biochem. 1976 Nov 15;70(2):403-8. doi: 10.1111/j.1432-1033.1976.tb11030.x.

DOI:10.1111/j.1432-1033.1976.tb11030.x

Abstract

Calf thymus histone H2B has been studied by means of 13c magnetic resonance. Most sidechain resonances have been assigned. Analysis of 13c peak intensities by a simple computer-aided method proves that 13c magnetic resonance spectroscopy can be used to monitor aggregation phenomena more accurately than 1H magnetic resonance or other physico-chemical methods. Re-examination of 1H spectral simulations shows substantial agreement with 13C results. It is concluded that in 0.1M added NaCL the H2b aggregates include the chain segment from 50 (+/-5) to 108 (+/-5).

摘要

已通过碳-13磁共振对小牛胸腺组蛋白H2B进行了研究。大部分侧链共振峰已被归属。通过一种简单的计算机辅助方法对碳-13峰强度进行分析，结果表明，与氢-1磁共振或其他物理化学方法相比，碳-13磁共振光谱法能够更准确地监测聚集现象。对氢-1光谱模拟结果的重新审视显示，其与碳-13的结果基本一致。得出的结论是，在添加了0.1M氯化钠的溶液中，H2B聚集体包含50（±5）至108（±5）的链段。