Michell R H, Pancake S J, Noseworthy J, Karnovsky M L
J Cell Biol. 1969 Jan;40(1):216-24. doi: 10.1083/jcb.40.1.216.
A method has been developed for measuring the rate of phagocytosis rather than the quantity of particles ingested per cell when the process is virtually complete. The method, which is simpler and more rapid than those described previously, utilizes cellular monolayers, radioactive particles, and short incubation times. Under the conditions described, the rate of uptake of particles by either guinea-pig peritoneal or human blood leukocytes was proportional to both cell concentration and the time of incubation, and was independent of changes in the concentration of particles during the measurement. The particles were retained by the cells for at least 90 min. The most suitable particles so far used have been (32)P-labeled Salmonella typhimurium, and acetyl-(14)C- or methyl-(14)C-labeled starch particles. The oxidation of (14)C-labeled glucose has been studied under the same conditions that were used for the assays of phagocytosis: the greatest increase in formation of (14)CO(2) from glucose-1-(14)C occurred a few minutes after the most rapid period of phagocytosis.
当吞噬过程基本完成时,已开发出一种用于测量吞噬速率而非每个细胞摄入颗粒数量的方法。该方法比先前描述的方法更简单、更快速,它利用细胞单层、放射性颗粒和短孵育时间。在所描述的条件下,豚鼠腹膜白细胞或人血白细胞摄取颗粒的速率与细胞浓度和孵育时间成正比,并且在测量过程中与颗粒浓度的变化无关。颗粒在细胞内保留至少90分钟。迄今为止使用的最合适的颗粒是(32)P标记的鼠伤寒沙门氏菌,以及乙酰-(14)C或甲基-(14)C标记的淀粉颗粒。已在用于吞噬作用测定的相同条件下研究了(14)C标记葡萄糖的氧化:从葡萄糖-1-(14)C形成(14)CO(2)的最大增加发生在吞噬作用最快速的时期之后几分钟。