Simberkoff M S, Elsbach P
J Exp Med. 1971 Dec 1;134(6):1417-30. doi: 10.1084/jem.134.6.1417.
The interaction, between mycoplasma (PPLO) and human or rabbit leukocytes was examined in vitro. Upon incubation of M. hominis or M. arthritidis for 2 hr with rabbit peritoneal exudate granulocytes or leukocytes from human peripheral blood, no killing of mycoplasma was observed either in the presence or absence of type-specific antiserum. However, (14)CO(2) production from glucose-1-(14)C was stimulated up to 10-fold in the presence of live or heat-killed PPLO. The extent of stimulation depended upon the number of organisms and the presence of type-specific antiserum. The stimulation of (14)CO(2) production seems not because of tight adherence of PPLO to the leukocytes, since PPLO were quantitatively recovered in the medium after sedimenting the granulocytes. The enhanced conversion of medium lysolecithin to cellular lecithin that accompanies phagocytosis of polystyrene particles was significantly reduced when PPLO were also present. Mycoplasma alone elicited no stimulation of lecithin formation. Killing of E. coli, a microorganism readily engulfed and killed by leukocytes in vitro, was diminished when the leukocytes were preincubated with mycoplasma. These findings indicate that M. hominis and M. arthritidis are not ingested by granulocytes to any detectable extent, but that these organisms affect the leukocytes' metabolism and also impair phagocytosis of E. coli.
对支原体（类胸膜肺炎微生物）与人或兔白细胞之间的相互作用进行了体外研究。将人型支原体或关节炎支原体与兔腹腔渗出粒细胞或人外周血白细胞孵育2小时后，无论有无型特异性抗血清，均未观察到支原体被杀灭。然而，在活的或热灭活的类胸膜肺炎微生物存在的情况下，从葡萄糖 -1-（14）C产生的（14）CO2产量可被刺激高达10倍。刺激程度取决于微生物数量和型特异性抗血清的存在。（14）CO2产量的刺激似乎不是因为类胸膜肺炎微生物紧密粘附于白细胞，因为在使粒细胞沉降后，类胸膜肺炎微生物在培养基中可被定量回收。当也存在类胸膜肺炎微生物时，伴随着聚苯乙烯颗粒吞噬作用的培养基溶血卵磷脂向细胞卵磷脂的转化增强显著降低。单独的支原体不会刺激卵磷脂形成。当白细胞与支原体预孵育时，大肠杆菌（一种在体外容易被白细胞吞噬并杀死的微生物）的杀灭作用减弱。这些发现表明，人型支原体和关节炎支原体在任何可检测的程度上都不会被粒细胞摄取，但这些微生物会影响白细胞的代谢，并且还会损害对大肠杆菌的吞噬作用。
