Krieger A G, Schiller N L, Roberts R B
Infect Immun. 1980 Jun;28(3):991-1000. doi: 10.1128/iai.28.3.991-1000.1980.
Utilizing monolayers of human polymorphonuclear leukocytes, optimal conditions for attachment and ingestion of Neisseria gonorrhoeae were determined. Both attachment and ingestion were optimal at 36 degrees C when a bacteria-leukocyte ratio of 100:1 was employed. After 30 min of incubation, log-phase viable type 2 gonococci were attached to 90% of leukocytes, whereas log-phase viable type 4 gonococci were ingested by 80 to 90% of cells. Respiratory inhibitors had no effect on attachment or ingestion, whereas glycolytic inhibitors blocked ingestion but did not affect attachment of gonocci to the leukocyte surface. Inhibition was dose dependent and partially reversible. The oxidative metabolism of leukocytes with gonococci attached or ingested was also examined. Attachment of log-phase type 2 gonococci stimulated a minimal increase in glucose oxidation and oxygen consumption by leukocytes in contrast to marked increases by leukocytes that had ingested viable type 4 or heat-killed typed 2 organisms. These results demonstrate that attachment of log-phase type 2 gonococci to the surface membrane does not stimulate significant leukocyte oxidative metabolism nor initiate the phagocytic process.
利用人多形核白细胞单层，确定了淋病奈瑟菌附着和摄取的最佳条件。当细菌与白细胞的比例为100:1时，在36℃下附着和摄取均达到最佳。孵育30分钟后，对数期存活的2型淋球菌附着于90%的白细胞，而对数期存活的4型淋球菌被80%至90%的细胞摄取。呼吸抑制剂对附着或摄取没有影响，而糖酵解抑制剂阻断摄取，但不影响淋球菌附着于白细胞表面。抑制作用呈剂量依赖性且部分可逆。还检查了附着或摄取淋球菌的白细胞的氧化代谢。与摄取了活的4型或热杀死的2型生物体的白细胞显著增加相比，对数期2型淋球菌的附着刺激白细胞的葡萄糖氧化和氧消耗最小增加。这些结果表明，对数期2型淋球菌附着于表面膜不会刺激显著的白细胞氧化代谢，也不会启动吞噬过程。
