大肠杆菌中细胞分裂的调控
Regulation of cell division in Escherichia coli.
作者信息
Pierucci O
出版信息
Biophys J. 1969 Jan;9(1):90-112. doi: 10.1016/S0006-3495(69)86371-X.
The rate of cell division was measured in cultures of Escherichia coli B/r strain after periods of partial or complete inhibition of deoxyribonucleic acid (DNA) synthesis. The rate of DNA synthesis was temporarily decreased by removing thymidine from the growth medium or replacing it with 5-bromouracil. After restoration of DNA synthesis, a temporary period of accelerated cell division was observed. The results were consistent with the idea that chromosome replication begins when an initiator complement of fixed size accumulated in the cell. The increase in the potential for the initiation of new replication points during inhibition of DNA synthesis results in an increase in the rate of cell division after an interval which encompasses the time for the arrival of these replication points to the termini of the chromosomes and the time from this event to division.
在部分或完全抑制脱氧核糖核酸（DNA）合成一段时间后，对大肠杆菌B/r菌株培养物中的细胞分裂速率进行了测量。通过从生长培养基中去除胸腺嘧啶核苷或将其替换为5-溴尿嘧啶，DNA合成速率会暂时降低。在DNA合成恢复后，观察到细胞分裂有一个暂时加速的阶段。这些结果与以下观点一致：当细胞中积累了固定大小的起始因子补充物时，染色体复制开始。在DNA合成受抑制期间，新复制起始点启动潜力的增加，会在一个包含这些复制点到达染色体末端所需时间以及从该事件到细胞分裂所需时间的间隔后，导致细胞分裂速率增加。
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