大肠杆菌中硫酸盐还原的正向调控。大肠杆菌K12无半胱氨酸突变体的分离、表征及定位
Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12.
作者信息
Jones-Mortimer M C
出版信息
Biochem J. 1968 Dec;110(3):589-95. doi: 10.1042/bj1100589.
Abstract
To determine to what extent the biosynthesis of cysteine in Escherichia coli resembles that in Salmonella typhimurium, the following experiments were performed. (1) Mutants of E. coli K 12 deficient in the biosynthesis of cysteine were isolated. (2) These mutants were classified by nutritional and biochemical criteria; some mutants lacked a single enzyme of sulphate reduction, other mutants appeared to lack two or more enzymes. (3) The genetic map predicted from the biochemical data alone is shown to be incorrect, and an alternative map, consistent with the genetic data, is proposed for the cys mutants of E. coli.
摘要
为了确定大肠杆菌中半胱氨酸的生物合成在多大程度上类似于鼠伤寒沙门氏菌中的生物合成，进行了以下实验。（1）分离出大肠杆菌K12中半胱氨酸生物合成缺陷的突变体。（2）根据营养和生化标准对这些突变体进行分类；一些突变体缺乏单一的硫酸盐还原酶，其他突变体似乎缺乏两种或更多种酶。（3）仅根据生化数据预测的遗传图谱被证明是不正确的，并且针对大肠杆菌的半胱氨酸突变体提出了与遗传数据一致的替代图谱。
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Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12.大肠杆菌中硫酸盐还原的正向调控。大肠杆菌K12无半胱氨酸突变体的分离、表征及定位
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