Brown L R, Dowell C E
J Virol. 1968 Nov;2(11):1290-5. doi: 10.1128/JVI.2.11.1290-1295.1968.
Techniques have been described for synchronization of bacteriophage M-13 infection of host cells. The latent period in infected cells was 10 min, and no appreciable number of intracellular phage was observed. Phage production proceeded in three phases after release of the starvation block: an initial rapid exponential rate of progeny phage release without cell lysis, a period of rate transition accompanying the resumption of host cell division, and a second, slower exponential rate of phage production which paralleled the rate of host cell division. The size of infected cells was not affected by infection, but the generation time was increased by 25%. Starved infected cells exhibited a much longer lag in attaining an exponential rate of growth upon the addition of nutrients than did an uninfected control culture.
已经描述了用于使噬菌体M-13感染宿主细胞同步化的技术。感染细胞中的潜伏期为10分钟，未观察到明显数量的细胞内噬菌体。在解除饥饿阻断后，噬菌体产生分为三个阶段：初始阶段是子代噬菌体快速指数释放且无细胞裂解，接着是伴随宿主细胞分裂恢复的速率转变期，最后是第二个较慢的指数产生速率阶段，该阶段与宿主细胞分裂速率平行。感染细胞的大小不受感染影响，但世代时间增加了25%。与未感染的对照培养物相比，饥饿感染的细胞在添加营养物质后达到指数生长速率的延迟时间长得多。
